ochratoxin A (OTA) also the mycotoxin citrinin has a kidney injurious and
possibly cancerogenic effect to humans. Nephritic tumors were observed in animal
tests. Citrinin together with OTA is produced by mold fungi (penicillia,
aspergilli) on numerous agricultural products and can endanger the health of
humans and animals.
recently, little attention was paid to citrinin as against OTA, which fact
possibly is due to the poorer analytical determinability. Therefore, the project
was aimed at the development of a reliable and sensitive HPLC method for the
determination of citrinin preferably in cereals and cereal products. A new
method was developed and validated which is based on the solid phase extraction
with polyamide columns and a gradient HPLC method with fluorescence detection.
The intensive consideration of the problem of citrinin analytics has resulted in
a method according to which citrinin can be determined in cereal samples with
satisfactory recovery rate and good detection sensitivity and precision.
Sufficient skill and experience with the method given, precise results, i.e.
variation coefficients <10 %, will be achieved. The mean recovery rates were
in the range 74-90 %. The detection limits of the new method determined
according to DIN 32645, at good precision (process standard deviation 4-7 %)
amounted to 1 µg/kg for wheat, rye, barley, maize, and oats.
analysis of several OTA containing samples according to the new method has shown
that citrinin is contained relatively frequently in brans, wheatings and shorts
containing a higher ratio of the outer layers of the grain kernel; beside OTA
citrinin also is contained in cocoa shells and raisins. Citrinin was detected in
14 of 35 OTA containing samples. Further, it was demonstrated that citrinin also
can be determined in red mold rice according to the new method.
method can be used for collecting data on the frequency of occurrence of
citrinin and consequently for increasing the consumer safety. The accreditated
test laboratory of IGV Ltd. is experienced and available for citrinin analyses.